Fig. 2

H. pylori SlyD upregulates TPT1 protein expression through hnRNPK and promotes GIM marker expression. A RNA-seq data from co-cultured AGS cells with H. pylori 26695 and △SlyD strain for 24 hours were integrated with two-dimensional gel electrophoresis mass spectrometry data from HpSlyD-GFP stable cell line to identify intersection genes hnRNPK. B The mRNA expression of hnRNPK was analyzed in H. pylori SlyD-stimulated GES-1 and AGS cells. GAPDH was used as internal control. C Western blot for the expression of hnRNPK in H. pylori SlyD-stimulated GES-1 and AGS cells. Protein band intensity was normalized to GAPDH band intensity. D RT-qPCR and western blot of hnRNPK and TPT1 expression, respectively, in H. pylori SlyD-stimulated GES-1 cells treated with sihnRNPK. Protein band intensity was normalized to GAPDH band intensity. E RT-qPCR and western blot of hnRNPK and TPT1 expression, respectively, in H. pylori SlyD-stimulated AGS cells with sihnRNPK treatment. Protein band intensity was normalized to GAPDH band intensity. F Western blot for the expression of GIM markers in H. pylori SlyD-stimulated GES-1 cells with sihnRNPK treatment. G The expression of GIM markers in H. pylori SlyD-stimulated AGS cells after sihnRNPK treatment was analyzed by western blot. Unless otherwise specified, protein band intensity was normalized to β-tubulin band intensity. Significance Levels: *P < .05, ** P < .01, *** P < .001, ns: non-significant