Fig. 2

Analysis of Treg trajectories and pathway enrichment in LUAD. A UMAP plot illustrating the identified Treg populations. B, D The trajectory distribution of each Treg population over time. The top represents the starting point of development, whereas the bottom is the developmental endpoint. C Heatmap of differently expressed genes along pseudotime. The colorbar on the left denotes different clusters for each gene. Rows: expression of differentially expressed genes; columns: cells shown in pseudotime order. E Pathway analysis of Treg clusters. F Boxplot delineating differentiation potential across different types of Tregs