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Fig. 4 | BMC Medicine

Fig. 4

From: Cryoablation-induced modulation of Treg cells and the TGF-β pathway in lung adenocarcinoma: implications for increased antitumor immunity

Fig. 4

Impact of cryoablation on Treg frequency in in vivo and in vitro experiments. A Effect of co-culture with PBMCs on Treg differentiation in the A549 and H1299 LUAD cell lines, wherein Group D exhibited the lowest Treg frequency. Representative plots are shown. A Healthy cells + complete medium, B healthy cells + necrotic medium, C sublethal cells + complete medium, D sublethal cells + necrotic medium). B Percentage of Tregs after co-culture with PBMCs. Data were pooled from 3 independent experiments. C Effect of cryoablation on Treg generation in the mouse spleen. Representative plots are shown. D Percentage of Tregs in the mouse spleen. n = 3 mice per group. Statistical significance between groups was assessed using the Brown-Forsythe and Welch ANOVA tests. *P < 0.05, **P < 0.01, and ***P < 0.001

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BMC Medicine

ISSN: 1741-7015

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