Fig. 1

Detection of CD10⁻ neuts in peripheral blood by flow cytometry. CD10⁻ neuts were defined as CD10⁻CD14⁻HLA-DR⁻CD33⁺CD45⁺SSC⁺ cells. The analysis method is outlined as follows: A FSC and SSC were used to exclude residual red blood cells and debris. B All nucleated cells (Gate B) were selected from Gate A based on SSC and CD45 expression. C CD33⁺CD45⁺SSC⁺ cells (Gate C) were isolated from Gate B. D CD14⁻CD33⁺CD45⁺SSC⁺ cells (Gate D) were selected from Gate C. E CD14⁻CD33⁺CD45⁺HLA-DR⁻ SSC⁺ cells (Gate E) were selected from Gate D. F CD10⁻CD14⁻HLA-DR⁻CD33⁺CD45⁺SSC⁺ cells, representing CD10⁻ neuts, were selected from Gate E. The percentage of CD10⁻CD14⁻HLA-DR⁻CD33⁺CD45⁺SSC⁺ cells in all neutrophils (Gate C) represented CD10⁻ neuts/neutrophils; the percentage of CD10⁻CD14⁻HLA-DR⁻CD33⁺CD45⁺SSC⁺ cells in nucleated cells (Gate B) represented CD10⁻ neuts/nucleated cells; the absolute count of CD10⁻ neuts was calculated as CD10⁻ neuts/nucleated cells × WBC count from the same-day routine blood tests. WBC, white blood cells