Fig. 6

Lrrk2 plays a role to alter the phosphorylation statu triggers mitochondrial fission (n = 3/group). A Sorted TPEX and TEX cells obtained from 16-week-old non-diabetic NOD mice were examined using qPCR to determine the expression of Dnm1l and Lrrk2. B Interaction network depicting between Dnm1l and Lrrk2 using the STRING database. C The Lrrk2 plasmid was used to transfect the 293 T cells. Cells were lysed for qPCR analyses. D The Lrrk2 plasmid was used to transfect the 293 T cells, followed by knockdown. Cells were lysed for qPCR analyses. E The 293 T cells were transfected with Lrrk2 plasmid or were transfected with Lrrk2 plasmid, followed by knockdown. Cells were lysed for immunoblotting analyses. F Western blot analysis was used to determine the protein expression in sorted TPEX and TEX cells that were separated from 8-week-old NY8.3 mice. G Sorted PD-1⁺CD8⁺ T cells in NY8.3 mice aged 6–8 weeks, stimulated them with anti-CD3 and anti-CD28 or IGRP_206–214 for 3 days, with or without the Midiv-1. Collected the cells and analyzed using Western blotting. ***P < 0.001, ****P < 0.0001. Mann–Whitney test (A, C,D). Data represent two independent experiments