Fig. 8

Single-cell RNA sequencing was performed on CD8⁺ T cells from Four-week-old NY8.3 mice 7 days after they were treated with anti-CD3mAb and IGRP_206–214. n = 2 biologically independent samples. A tSNE clusters of cells from the scRNA-seq. B Dot plot examination of specific markers that reflect TPEX and TEX cell subsets; dot size and color intensity indicated the percentage of cells expressing the gene and the z-score mean expression, respectively. C Normalized signaling pathway expression projected onto UMAP clusters. D Slingshot analysis was used to predict developmental trajectories. Color-coding of cells was based on pseudotime. E Cellcell communication analysis by Cellcall. F Bubble plots showed ligand-receptor pairs of different CD8⁺T cell subsets in different treated groups