Diagnosis of celiac disease on a gluten-free diet: a multicenter prospective quasi-experimental clinical study

Table 4 Comparison of advantages and disadvantages of the different methodological approaches presented

	CD8⁺ T-cell	IL-2	% TCRγδ⁺	*UBE2L3*
Patient requirements
Strict GFD	Yes	Yes	No	Yes
GC requirement	Yes	Yes	No	No
GC duration	3 days	1 day	–	–
Intervention	Minimally invasive (blood)	Minimally invasive (blood)	Invasive (upper endoscopy, biopsy)	Minimally invasive (blood)
Procedure delay	Minimal	Minimal	Endoscopy waiting list	Minimal
Visits for sample collection	2 (baseline and day 6)	2 (baseline and 4 h)	1	1
Technical requirements
Processing time/sample	2.5 h	9 h	3 h	6 h
Special requirement	Flow cytometer	Ultrasensitive immunoassay platform	Flow cytometer	Real-time PCR
Minimal sample size for cost-effectiveness	1 patient	10 patients	1 patient	1 patient^a
Time to diagnostic results	6 days	2 days^b	1 day	1–2 days^b

GFD gluten-free diet, GC gluten challenge
^aIt may depend on the center of analysis. ^bThe number of patients for a cost-effective analysis may cause considerable delay

ISSN: 1741-7015