Fig. 4

Validation of differentially expressed PIWIL1 in different datasets. A–D The expression of PIWIL1 in EOCRC and LOCRC patients from the TCGA (A), GSE39582 (B), GSE17536 (C), and GSE17537 (D) datasets. E–G Differential expression analysis of PIWIL1 between normal and EOCRC (E) or LOCRC (F) tissues from the TCGA cohort and clinical specimens (G). Significance was determined using a Wilcoxon rank-sum test. H,I UMAP plots of unsupervised clustering result of tumor subclusters in EOCRC (H) and LOCRC (I) from the GSE132465 dataset. The tSNE plots show single cells of CRC tissues across six major cell types. J,K The expression pattern of PIWIL1 in single-cell level in EOCRC (J) and LOCRC (K) tissues. Cells expressing PIWIL1 are colored red in the tSNE plots using the normalized value. L–M Bubble plots of the average and percent expression of PIWIL1 in different cell subtypes of EOCRC (L) and LOCRC (M) tissues. EOCRC, early-onset colorectal cancer; LOCRC, late-onset CRC; tSNE, t-distributed stochastic neighbor embedding